Fig. 7

ATF5 knockdown reversed damage induced by continuous HG exposure in HK-2 cells, while overexpression ATF5 exacerbated these effect. A Western blot analysis to confirm ATF5 plasmid transfected efficiency. A1 Relative band density of ATF5. B Immunofluorescence analysis were performed to access ATF5 nuclear translocation in HK-2 cells. Scale bar: 2 μm. C Western blot analysis of ATF5, HSP60, LONP1, FN, C-CAS3 expression in whole cell lysate. C1–C4 Relative band density. D Western blot analysis of HSP60, LONP1 mitochondrial proteins. D1–D2 Relative band density. E Western blot analysis of ATF5, FN, C-CAS3 in whole cell lysate. E1–E2 Relative band density. F MitoSOX dye was used to detect mitochondrial ROS. Scale bar: 30 μm. G Representative microscopy images of FITC-Annexin staining. Scale bar: 30 μm. F1–G1 Semiquantification of Relative fluorescence intensity, the integrated optical density of MitoSOX/DAPI and FITC-Annexin V/DAPI were calculated by IPP. All data are presented as means ± SD; *p < 0.05 vs LG; ^#p < 0.05 vs 24 h HG. ^@p < 0.05 vs 24 h HG + ATF5-siRNA. n = 3