Fig. 8

ATF5 plasmids and HSP60-siRNA transfection blocked the negative effect of ATF5 on HK-2 cells with continuous HG treatment. A Western blot analysis of ATF5, HSP60, LONP1, FN, C-CAS3 expression in HK-2 cells transfected with HSP60-siRNA alone. A1 Relative band density. B Western blot analysis of ATF5, HSP60, LONP1, FN, C-CAS3 expression in HK-2 cells transfected with ATF5 plasmid and HSP60-siRNA. B1 Relative band density. C MitoSOX dye was used to detect mitochondrial ROS. Scale bar: 30 μm. D Representative microscopy images of FITC-Annexin staining. Scale bar: 30 μm. C1–D1 Semiquantification of Relative fluorescence intensity, the integrated optical density of MitoSOX/DAPI and FITC-Annexin V/DAPI were calculated by IPP. All data are presented as means ± SD; *p < 0.05 vs LG; ^#p < 0.05, ^##p < 0.01 vs 24 h HG + Neg-siRNA + OE-NC. n = 3