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Fig. 8 | Molecular Medicine

Fig. 8

From: CRISPR-cas9 screening identified lethal genes enriched in Hippo kinase pathway and of predictive significance in primary low-grade glioma

Fig. 8

RPE65 in vitro. A The expression level of RPE65 in different cell lines. qRT-PCR (B) and western blot (C) was used to analyse the expression level of RPE65 in transfection by sh-NC or sh-RPE65 for 24 h. D Cell viability of U87 or T89G cells after knocking down RPE65 was determined using CCK8 assays. E Invasion ability of cells after knocking down RPE65 was determined using transwell assays and quantify in T98G and U87 cells with box plots. F Migration ability of cells after knocking down RPE65 was determined using wound healing assays and quantify in T98G and U87 cells with box plots (magnification and wound healing: ×100). *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. Error bars indicate mean ± SD

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Molecular Medicine

ISSN: 1528-3658