Fig. 4
From: TRIM37 exacerbates hepatic ischemia/reperfusion injury by facilitating IKKγ translocation
TRIM37 increased the K63 chain by directly binding to TRAF6. A Alignment of multispecies homologous amino acid sequences of TRIM37 and its conserved domains. B Effectiveness after the inhibition of TRAF2, TRAF4 and TRAF6 in vitro. C The interaction between TRAF6 and TRIM37 was detected by immunoprecipitation after TRAF6 inhibition in vitro. D Determination of TRAF6-guided K63 ubiquitin. E The interaction between TRAF6 and TRIM37 was detected by immunoprecipitation after TRIM37 overexpression in vitro. F Determination of TRAF6-guided K63 ubiquitin in the presence of TRIM37 overexpression. G Immunofluorescence analysis of p-IKKβ and F4/80 in primary Kupffer cells after H/R exposure, scale bar = 50 μm for immunofluorescence. H Protein expression of TAK1 when TRAF6 was suppressed in vitro. I Cell cytokines and chemokines after suppressing TRAF6. J, K The lack of TRAF6 inhibited hepatic pathological changes and apoptosis in liver I/R injury under the condition of TRIM37 upregulation. Scale bar = 200 μm for HE, scale bar = 100 μm for TUNEL. *p < 0.05