Fig. 7
From: TRIM37 exacerbates hepatic ischemia/reperfusion injury by facilitating IKKγ translocation
TRAF6 prolonged the inflammatory phase by stabilizing the IKK complex in vitro. A Inhibition of TRAF6 in vitro has a stronger anti-inflammatory effect than ubiquitin repression by C25-140. The protein expression of IKK/IκBα/NF-κB was detected at 6/24 h after H/R. B The protein expression of IKK catalytic and regulatory subunits was detected at 6/24 h after H/R. C The translocation of IKKγ was monitored at 6/24 h after H/R. D Immunofluorescence analysis of IKKγ and F4/80 in primary Kupffer cells after H/R treatment, scale bar = 50 μm for immunofluorescence. E, F Changes in the expression of IL-10 and CXCL-10 in the cell supernatant from 1 to 36 h after reoxygenation with TRIM37 amplification and TRAF6 deficiency. Two lanes in a row are from the same mixed samples, the in vitro experiment was repeated independently at least 3 times