Fig. 1

CIRP was induced by DHCA and Cirp knockout impaired renal function after DHCA. A Schematic illustration of experimental time and temperature flow of rat DHCA model. B Level of Cirp/β-actin mRNA in kidneys from the sham, DHCA and DHCA + Cirp^−/− groups (n = 5 per group). C Representative image of western blot analysis of CIRP in renal tissues from the three groups. D The ratio of CIRP to β-actin by western blot analysis. E Immunofluorescence staining of kidneys from three groups (original magnification, × 400). F The CIRP-positive cells were quantified by the percentage in 5 randomly selected microscopic vision fields. (G) Postoperative serum creatinine (SCr), blood urea nitrogen (BUN), uric acid and cystatin C in the three groups. H Levels of Kim-1/β-actin, Ngal/β-actin and cystatin C/β-actin mRNA in kidneys from the three groups. I ELISA analyses of renal KIM-1 and NGAL in the three groups. J Postoperative urine (IGFBP-7) × (TIMP-2) in the DHCA and the DHCA + Cirp^−/− groups (n = 5 per group). Statistical differences between two groups were compared using Student’s t test, and comparisons among no less than three groups were examined by one-way analysis of variance (ANOVA) followed by the Tukey test. *P < 0.05, **P < 0.01, ***P < 0.001