Fig. 2

Cirp deficiency intensified DHCA-induced renal injury. A Representative H&E staining images of the renal cortex and medulla in the sham, DHCA and DHCA + Cirp^−/− groups (original magnification, × 200). Black arrows, exfoliation of renal tubular epithelial cells and cellular cast formation. Blue arrows, glomerular pyknosis and disintegration. B Pathological lesion score of renal samples from three groups. C Representative TEM images of glomeruli and renal tubule from three groups (original magnification, × 2500). Black arrows, karyopyknosis. Yellow arrows, thickening of the glomerular endothelial basement membrane. Red arrows, partial fusion of foot process. Green arrows, mitochondrial edema, mitochondrial electron density decrease and mitochondrial crest loss. Purple arrows, mitochondrial rupture. Blue arrows, brush border structure disorder. Asterisk, apoptotic body. D TUNEL staining of kidneys from the three groups (original magnification, × 400). E Apoptotic degree was counted as the percentage of TUNEL signal in 5 randomly selected fields. Statistical significance was examined by one-way analysis of variance (ANOVA) followed by the Tukey test. **P < 0.01, ***P < 0.001