Fig. 5

Cirp knockout exacerbated the mitochondrial apoptotic pathway and the death receptor apoptotic pathway after DHCA. A Western blot analyses of Bcl-2, Bax, AIF, Apaf-1, caspase 9 and cleaved caspase 9, FADD, caspase 8 and cleaved caspase 8 and caspase 3 and cleaved caspase 3 in kidneys from the sham, DHCA and DHCA + Cirp^−/− groups. B The ratio of key targets in the mitochondrial apoptotic pathway to β-actin by western blot analysis. C Representative immunohistochemistry images of cleaved caspase 3 in the renal cortex and medulla from the three groups (original magnification, × 200). D The ratio of key targets in death receptor apoptotic pathway to β-actin by western blot analysis. E The cleaved caspase 3-positive cells were quantified by the percentage in 5 randomly selected microscopic vision fields. Statistical significance was examined by one-way analysis of variance (ANOVA) followed by the Tukey test. *P < 0.05, **P < 0.01, ***P < 0.001