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Fig. 6 | Molecular Medicine

Fig. 6

From: CIRP attenuates acute kidney injury after hypothermic cardiovascular surgery by inhibiting PHD3/HIF-1α-mediated ROS-TGF-β1/p38 MAPK activation and mitochondrial apoptotic pathways

Fig. 6

Apoptosis of HK-2 was aggravated by CIRP knockdown but attenuated by PHD3 knockdown. A TUNEL staining of HK-2 in the control, OGD, OGD + siNC, OGD + siCIRP, OGD + siPHD3 groups (original magnification, × 400). Yellow arrows, apoptotic body. B The apoptotic degree was counted as the percentage of TUNEL signal in 5 randomly selected fields. C, D Apoptosis rate measurement by flow cytometry with Annexin V-FITC/propidium iodide (PI) double staining of HK-2 in five groups. E The cell vitality detection by cell counting kit (CCK)-8 of HK-2 in five groups. Statistical significance was examined by one-way analysis of variance (ANOVA) followed by the Tukey test. *P < 0.05, **P < 0.01, ***P < 0.001

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Molecular Medicine

ISSN: 1528-3658