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Fig. 7 | Molecular Medicine

Fig. 7

From: CIRP attenuates acute kidney injury after hypothermic cardiovascular surgery by inhibiting PHD3/HIF-1α-mediated ROS-TGF-β1/p38 MAPK activation and mitochondrial apoptotic pathways

Fig. 7

Enarodustat reversed HK-2 apoptosis aggravated by CIRP knockdown and attenuated HK-2 apoptosis in hypothermic OGD. A Experimental protocol of siRNA transfection and enarodustat or vehicle pretreatment of HK-2 cells. B, C Western blot analyses of CIRP, PHD3, HIF-1α, Bax, Bcl-2, caspase 9 and cleaved caspase 9, FADD, caspase 8 and cleaved caspase 8, caspase 3 and cleaved caspase 3 and β-actin of HK-2 in the OGD, OGD + siCIRP, OGD + enarodustat and OGD + siCIRP + Enarodustat groups. D, E Apoptosis rate measurement by flow cytometry with Annexin V-FITC/propidium iodide (PI) double staining of HK-2 in four groups. Statistical significance was examined by one-way analysis of variance (ANOVA) followed by the Tukey test. *P < 0.05, **P < 0.01, ***P < 0.001

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Molecular Medicine

ISSN: 1528-3658