Fig. 3

Hcy treatment triggers pyroptosis in THP-1. A Increases in the expression of pyroptosis-related genes (NLRP3 and IL-1β) at mRNA levels by different concentrations Hcy of 0, 1, 5, and 10 mM. B Western blot was used to evaluate the pyroptosis-related proteins level in different groups. C–G Relative protein expressions quantification of pyroptosis-related proteins. H Cell viability detected by CCK8. I The cell death was measured with Hoechst 33342 (blue)/PI (red) double-fluorescent staining. The scale bars correspond to 100 μm. J The levels of LDH were measured by commercial kits. K The morphology of cells was observed with scanning electron microscopy (SEM). The scale bars correspond to 10 μm. The data are shown as the mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001