Fig. 4

Caspase-1 inhibitor represses Hcy-induced macrophage pyroptosis. Macrophages were pretreated with caspase-1 inhibitor (VX-765, 10 μM) for 1 h, and then the cells were incubated with Hcy (10 mM) for 24 h. A Western blot was used to evaluate the pyroptosis-related proteins level in different groups. B–E Quantitative analysis of pyroptosis-associated protein expression. F Cell viability detected by CCK8. G The cell death was measured with Hoechst 33,342 (blue)/PI (red) double-fluorescent staining. The scale bars correspond to 100 μm. H LDH assay was used to evaluate the cell membrane integrity. I The morphology of cells was observed with scanning electron microscopy (SEM). The scale bars correspond to 10 μm. The data are shown as the mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001