Fig. 7
Hcy increases ER-mitochondria colocalization. A Western blot was used to evaluate the MAM-related proteins (p-IP3R, VDAC, mfn-2) level after Hcy treated. B–D Relative protein expressions quantification of MAM-related proteins. E The ER was stained with ER-tracker (shown in green), and mitochondria were stained with mito-tracker (shown in red) and then imaged using confocal microscopy. Colocalization was shown in yellow in the merged images. The scale bars correspond to 30 μm. F Ca2+ change with the fluorescence. The levels of cellular and mitochondrial calcium were measured by Fluo-4, AM and Rhod-2, AM, respectively. The scale bars correspond to 100 μm. G Western blot was used to evaluate the pyroptosis-related protein level after calcium chelator BAPTA pretreatment. H–K Relative protein expressions quantification of pyroptosis-associated proteins. The data are shown as the mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001