Fig. 4
From: Nr4a1 promotes renal interstitial fibrosis by regulating the p38 MAPK phosphorylation
Nr4a1 promotes TGF-β1-induced fibrotic effect in HK-2 cells (A) Western blot analysis of Nr4a1, Fn, Col-I expression in HK-2 cells with or without TGF-β1. (B) Quantitative analysis of the expression of targeted proteins in (A). (C) qRT-PCR analysis of the expression of Nr4a1 mRNA in HK-2 cells transfected with Nr4a1 siRNA or NC siRNA. (D) Western blot analysis of the expression of Nr4a1 mRNA in HK-2 cells transfected with Nr4a1 siRNA or NC siRNA. (E) Quantitative analysis of the expression of targeted proteins in (D). (E) Western blot analysis and (F) corresponding quantitative analysis of Fn and Col-I expression in HK-2 cells transfected with Nr4a1 siRNA after TGF-β1 treatment. (G) Western blot analysis and (H) corresponding quantitative analysis of Nr4a1, Fn, Col-I expression in Hk-2 cells with or without 10ug/ml Csn-B treatment. Statistical differences between two groups were determined by the unpaired t test. And one-way analysis of variance (ANOVA), followed by Tukey’s post-tests, was used to determine the statistical differences among groups. *p<0.05, **p<0.01, ***p<0.001