Fig. 5

Rad1 attenuates DNA double-strand breaks (DSBs) and cell cycle arrest in AECII. RLE-6TN cells were randomly divided into five groups: a non‐interference air group (Control + 48hAir), a non‐interference hyperoxia group (Control + 48hO2), a blank-transfection hyperoxia group (NC + 48hO2), a siRNA-Rad1 transfection hyperoxia group (siRNA-Rad1 + 48hO2), and a Rad1 overexpression hyperoxia group (OE-Rad1 + 48hO2). A Immunofluorescence detection of γ-H2AX expression in RLE-6TN cells transfected with Rad1 overexpression or knockdown constructs. (Scale bar = 10 μm, Red fluorescence-labeled γ-H2AX, blue fluorescence-labeled DAPI represent nucleus). B Comet assay of DNA damage in RLE-6TN cells transfected with Rad1 overexpression or knockdown constructs (Scale bar = 20 μm). C–E Western blot detection of γ-H2AX and p21 expression in RLE-6TN cells transfected with Rad1 overexpression or knockdown constructs (C = control, M = model). F The comet tail length, the olive tail moment, and the percentage of tail DNA content in RLE-6TN cells transfected with Rad1 overexpression or knockdown constructs