Fig. 2
From: Epigenetic regulatory mechanism of ADAMTS12 expression in osteoarthritis
STAT1 regulates ADAMTS12 transcription. Rat chondrocytes were transfected with NC or shSTAT1, followed by IL-1β incubation. Cells were grouped as control, IL-1β, NC + IL-1β, or shSTAT1 + IL-1β. (A) ChIP-qPCR analysis for binding of STAT1 on ADAMTS12 promoter in rat chondrocytes stimulated with or without IL-1β. n = 3 (B) The binding of STAT1 on ADAMTS12 promoter in rat chondrocytes through EMSA assay. n = 3 (C-D) RT-qPCR and western blot assays for STAT1 and ADAMTS12 levels in rat chondrocytes. n = 3 (E) TUNEL staining of rat chondrocyte. n = 3 (F) Apoptosis of rat chondrocyte detected by flow cytometry. n = 3. *P < 0.05; **, P < 0.01