Fig. 4
From: Epigenetic regulatory mechanism of ADAMTS12 expression in osteoarthritis
METTL3 regulates STAT1 expression. (A) MeRIP-qPCR assay for m6A methylation level of STAT1 in cartilage tissues of normal cases or patients with OA. n = 5. (B–D) METTL3 levels in cartilage tissues of normal or patients with OA by IHC, RT-qRCR, and western blot assays. n = 5. (E) MeRIP-qPCR assay for m6A methylation level of STAT1 in cartilage tissues in sham or OA rat model groups. n = 5. (F–H) METTL3 levels in cartilage tissues in sham or OA rat model groups by IHC, RT-qRCR, and western blot. n = 5. (I) MeRIP-qPCR assay for m6A methylation level of STAT1 in rat chondrocytes transfected with NC or shMETTL3 before IL-1β stimulation. n = 3. (J–K) The mRNA and protein expression levels of STAT1 in rat chondrocytes by qPCR and western blot assays. n = 3. *P < 0.05; **, P < 0.01; ***, P < 0.001