Fig. 5
From: Epigenetic regulatory mechanism of ADAMTS12 expression in osteoarthritis
METTL3/IGF2BP2 axis regulates STAT1 stability and expression. (A–B) RIP assay for binding of IGF2BP2 and STAT1 in patients with OA and rat models. n = 5. (C) RIP assay for binding of IGF2BP2 and STAT1 in IL-1β-treated rat chondrocytes. n = 3. (D) STAT1 stability detected via RT-qPCR in IL-1β-treated rat chondrocytes transfected with NC or shIGF2BP2 through actinomycin D assay. n = 3. (E-F) The mRNA and protein levels of STAT1 and ADAMTS12 in IL-1β-treated rat chondrocytes transfected with OE-NC, OE-METTL3, OE-METTL3 + NC, or OE-METTL3 + shIGF2BP2 by RT-qPCR and western blot. n = 3. (G) TUNEL staining of rat chondrocyte. n = 3 (H) Apoptosis of rat chondrocyte detected by flow cytometry. n = 3. *P < 0.05; **, P < 0.01; ***, P < 0.001