Fig. 6
From: Epigenetic regulatory mechanism of ADAMTS12 expression in osteoarthritis
METTL3 regulates IL-1β-treated chondrocytes by modulating ADAMTS12. Rat chondrocytes were transfected with NC, shMETTL3, shMETTL3 + OE-NC, or shMETTL3 + OE-ADAMTS12 followed by IL-1β stimulation. (A-B) RT-qPCR and western blot for METTL3 and ADAMTS12 expressions in IL-1β-treated rat chondrocytes. n = 3. (C) IF assays for METTL3, STAT1, and ADAMTS12 levels in treated rat chondrocytes. n = 3 (D) TUNEL staining of rat chondrocyte. n = 3 (E) Apoptosis of rat chondrocyte detected by flow cytometry. n = 3. *P < 0.05; **, P < 0.01; ***, P < 0.001