Fig. 7
From: Epigenetic regulatory mechanism of ADAMTS12 expression in osteoarthritis
METTL3 regulates osteoarthritis progression in OA rats via mediating ADAMTS12. OA rats were induced by the ACL-T method and then treated with METTL3 inhibitor SAH. Rats were grouped as sham, ACL-T, and ACL-T + SAH. (A) HE, PAS, and safranin O-fast green of rat cartilage tissues in each group. n = 5 (B and C) OARSI score and micro-CT assays were performed in each group. n = 5. (D–F) RT-qPCR, western blot, and IHC assays for METTL3, ADAMTS12, and STAT1 levels in rat cartilage tissues in each group. n = 5. (G) The binding relationship between STAT1 and ADAMTS12 promoter region in rat cartilage tissues was tested by ChIP assay. n = 5. (H) The binding relationship between STAT1 mRNA and IGF2BP2 in rat cartilage tissues was tested by RIP assay. n = 5. *P < 0.05; **, P < 0.01; ***, P < 0.001