Fig. 4

DYNLT1 regulates ATP levels and mitochondrial membrane potential in breast cancer cells

A A volcano plot represents the genes correlated with DYNLT1: genes with significant negative correlation and positive correlation were navy and red respectively and genes with no significant correlation are colored gray. B Gene Ontology (GO) analysis revealed the enriched biological process (BPs), cellular components (CCs) and molecular functions (MFs) involved in top 500 genes that significantly correlated with DYNLT1. C Immunofluorescence results showed that DYNLT1 was co-localized with mitochondria. DYNLT1 (green), mitochondria (Red), nuclei (bule). Scale bar represents 2 μm. D-E The cellular ATP levels in stable DYNLT1 knockdown MCF-7 (D) or MDA-MB-468 (E) breast cancer cells. F-G Mitochondrial membrane potential in stable DYNLT1 knockdown MCF-7 (F) or MDA-MB-468 (G) breast cancer cells. *, p < 0.05; ***, p < 0.0001; ****, p < 0.0001. Scale bar represents 10 μm. All experiments were performed at least three replicates