Fig. 5

DYNLT1 colocalizes with VDAC1 in mitochondria to promote mitochondrial metabolism

A Protein interaction analysis from STRING database. B Differential expression of VDAC1 in breast tumor tissues and normal tissues. C Survival rate of breast cancer patients in high and low VDAC1 expression group. D-E WB results showed that protein expression of GAPDH, DYNLT1 and VDAC1 in MCF-7 (D) or MDA-MB-468 (E) breast cancer cells with indicated treatment. F-G Co-IP results showed that DYNLT1 can interact with VDAC1 in MCF-7 (F) or MDA-MB-468 (G) breast cancer cells. H Immunofluorescence results showed that DYNLT1 was co-localized with VDAC1. DYNLT1 (green), VDAC1 (Red), DAPI (bule). Scale bar represents 2 μm. I The cellular ATP levels with indicated treatment in MCF-7 cells. The figure on the right shows the protein expressions of GAPDH, DYNLT1 and VDAC1 with indicated treatment. *, p < 0.05; **, p < 0.01; ***, p < 0.001, ****, p < 0.0001. All experiments were performed at least three replicates