Table 1 Primers used for the PCR amplification
Gene | Accession number | primer sequence | |
|---|---|---|---|
18S rRNA (Reference gene) | NR_046237.2 | F: | GTAACCCGTTGAACCCCATT |
R: | CAAGCTTATGACCCGCACTT | ||
PGC-1α | NM_031347.1 | F: | GTGCAGCCAAGACTCTGTATGG |
R: | GTCCAGGTCATTCACATCAAGTTC | ||
NRF-1 | NM_001100708.1 | F: | TTACTCTGCTGTGGCTGATGG |
R: | CCTCTGATGCTTGCGTCGTCT | ||
Tfam | NM_031326.2 | F: | CCCACAGAGAACAGAAACAG |
R: | CCCTGGAAGCTTTCAGATACG | ||
POLG | NM_053528.1 | F: | GGACCTCCCTTAGAGAGGGA |
R: | AGCATGCCAGCCAGAGTCACT | ||
mtDNA | NC_040919.1 | F: | AATGGTTCGTTTGTTCAACGATT |
R: | AGAAACCGACCTGGATTGCTC | ||
n-PGC-1α | NM_031347.1 | F: | ATGAATGCAGCGGTCTTAGC |
R: | AACAATGGCAGGGTTTGTTC | ||
DRP1 | NM_053655.3 | F: | GATGCCATAGTTGAAGTGGTGAC |
R: | CCACAAGCATCAGCAAAGTCTGG | ||
OPA1 | NM_133585.3 | F: | CAGCTGGCAGAAGATCTCAAG |
R: | CATGAGCAGGATTTTGACACC | ||
MFN1 | NM_130894.4 | F: | GCCAGCTTCCTTGAAGACAC |
R: | GCAGAACTTTGTCCCAGAGC | ||
NF-κB (P65) | NM_199267.2 | F: | CAGGACCAGGAACAGTTCGAA |
R: | CCAGGTTCTGGAAGCTATGGAT | ||