Fig. 1

Screening for myelination-related TFs by analyzing the transcriptomic data of myelinating SCs. A Graphs (top) showing module eigengene expression trends. Heatmaps (middle) depicting expression of genes (columns) across samples (rows) for eight time-specific modules (purple and turquoise correspond to 0 and 1 day; green, brown, yellow, cyan s and salmon correspond to 3, 7 and 14 days; blue correspond to 14 and 21 days). The colors, from blue through white to red, indicate low to high correlations. GO analyses (bottom) showing the main biological function of the eight modules. Schematic diagram indicates the time-specific modules of SC lineage development and myelination. The gray dotted line divides it into three parts, namely SC proliferation and adhesion (0–1 day), SC differentiation, surrounding axons and myelination (3–14 days), and forming mature myelin sheath (14–21 days); red thick curved arrows indicate cell proliferation; red thin curved arrows indicate SCs wrapping axons. B Heat map of the changes in the expression of 15 signature TFs (rows) during SC myelination (columns). The red dotted box indicates 3 genes with the same expression trend. C QPCR analysis of 6 gene expression dynamic in SCs during myelination. One-way ANOVA, ***p < 0.001, n = 3 per group