Fig. 3
Expression changes and cellular localization of Stat1 after nerve injury. A (a1) Western blots showing the expression change of Stat1, MAG, Nab2 and Egr2 in nerve injury segment at the indicated time points following a nerve crush, with uninjured nerve used as the control (Ctrl). GAPDH served as the loading control. (a2–6) Histogram quantitatively compare the expression changes of Stat1, MAG, Nab2, and Egr2. ###p < 0.001, one-way ANOVA, *p < 0.05, **p < 0.01, ***p < 0.001 vs control, n = 3 ~ 4 per group. B Immunofluorescence staining of S100β (a marker of SCs, red) and Stat1 (green) in injured sciatic nerve showing cellular localization of Stat1: on day 1 of injury, co-localization of Stat1 and S100β was almost absent; on day 4 of injury, there was a small amount of Stat1 and S100β co-localization; and from day 7 to day 28 of injury, Stat1 was mainly localized in SCs. Scale bars, 100 μm