Fig. 4

Specific knockdown Stat1 in SCs inhibits remyelination. A Western blots showed that Stat1 expression was down-regulated by AAV-CNP-Stat1-shRNA 21 days after virus injection (on the left of the red dotted line), and MAG and Stat1 levels were down-regulated in AAV-CNP-Stat1-shRNA-treated regenerated nerves 28 days after injury relative to control (on the right of the red dotted line), with GAPDH as the internal standard. T-test, *p < 0.05, **p < 0.01 vs control (AAV-CNP-Scramble), n = 3 per group. B MAG (red) immunostaining shows myelin sheaths in regenerated nerves treated with AAV-CNP-Stat1-shRNA and AAV-CNP-Scramble. Green is EGFP on AAV. Scale bar, 100 μm. High-magnification images of boxed regions are also shown, scale bar, 50 μm. Histograms comparing the density of myelin sheaths. T-test, *p < 0.05 vs AAV-Scramble, n = 3 per group. C. Representative transmission electron micrographs of the regenerated nerve treated with AAV-CNP-Stat1-shRNA and AAV-CNP-Scramble. Scale bars, 5 μm. Zoomed in, the high magnification image Scale bars, 0.5 μm. Histograms comparing the G-ratio, axon diameter, myelin sheath layers and thickness. T-test, *p < 0.05, **p < 0.01 vs AAV-Scramble; ns, not significant, n = 3 per group