Fig. 5

Knockdown Stat1 in SCs inhibits myelination in a DRG neurons and SCs co-culture system. A. Schematic showing the co-culture system; (a1) Representative phase-contrast microscopy images shows the myelin sheaths after co-culture for 21 days. Zoomed in shows high magnification image. Red arrow, myelin segments. Scale bars, 20 μm; (a2) MAG (red) and NF200 (green) immunostaining for 21 days of co-culture showed the production of numerous myelin segments. Zoomed in shows high magnification image. Scale bar, 50 μm. B MAG (green) and NF200 (red) immunocytochemistry indicated that knockdown Stat1 in SC with LV-Stat1-shRNA inhibits myelination co-culture systems. Zoomed in shows the high magnification images of white boxes. Scale bars, 50 μm. C Western blots compares the MAG and Stat1 levels in the myelin co-culture system at day 21 of Stat1 knockdown or control SCs co-culture with neurons. T-test, **p < 0.01 vs LV-Scramble, n = 3 per group