Fig. 6

Knockdown Stat1 in SCs reduces SC differentiation. A (a1) Representative phase contrast microscopy images and P0 (green) and MAG (red) immunocytochemical images of SCs before and after differentiation. Scale bar, 100 μm; (a2) Western blots comparison of MAG and P0 levels in differentiated SCs at 24, 48 and 72 h, and vehicle as the control. One-way ANOVA, *** p < 0.001, n = 3 per group. B (b1) Western blots comparison of Stat1 levels in differentiated SCs at 24, 48 and 72 h, and vehicle as the control. One-way ANOVA, *** p < 0.001, n = 3 per group; (b2) Western blots showing the expression level of Stat1 and MAG in differentiated SCs treated with Stat1-shRNA and Scramble. The histograms showing that knockdown Stat1 reduces MAG expression in differentiated SCs. T-test, **p < 0.01 vs Scramble, n = 3 per group