Fig. 7

Stat1 knockdown in SCs enhances SC migration and SC proliferation. A Knockdown Stat1 in SCs promotes SC Differentiation. (a1) An insert-based scratch migration assay (n = 12, t-test, *** p < 0.001 vs Scramble) and (a2) a migration assay of cell spheroids growing on fascicular DRG axons (n = 50, t-test, *** p < 0.001 vs Scramble) showing SCs transfection with Stat1-siRNA have higher migratory capacity than cells transfected with a scramble. Scale bars, 100 μm. Red dashed circles indicate the extent of SC migration. Scale bars, 100 μm. B Knockdown Stat1 in SCs promotes SC proliferation. Results showing that SCs transfected with Stat1-siRNA growing on PDL (b1; n = 6, t-test, **p < 0.01 vs Scramble) or on fascicular DRG axons (b2, n = 5, t-test, ***p < 0.001 vs Scramble) have the higher proliferation ratio than that of scramble treated SCs. Green dots, proliferating SCs; blue dots, all cell nuclei; and red, NF200, fascicular DRG axons. Scale bar, 100 μm