Fig. 8

Knockdown Stat1 in SCs enhance the expression of factors negatively regulating SC differentiation. A Volcano plot showing the transcriptome profiles of differentiated SCs treated with Stat1-siRNA and Scramble. Red dots, upregulated genes (p < 0.05, fold-change > 1.5); green dots, downregulated genes (p < 0.05, fold-change > 1.5). B Heatmap of representative differentially expressed genes and their functional classification. C Downregulated and upregulated genes in differentiated SCs treated with Stat1-siRNA and Scramble. D qPCR showing that genes related to SC differentiation decreased (up) and increased (down) in differentiated SCs treated with Stat1-siRNA relative to Scramble. T-test, *p < 0.01, **p < 0.01, ***p < 0.01 vs Scramble, n = 3 per group. E GSEA enrichment scores (|NES|> 1, NOM p-value < 0.05, FDR q-value < 0.25) for cell differentiation, cell cycle, cell adhesion and lipoprotein biosynthetic process gene sets in in differentiated SCs treated with Stat1-siRNA and Scramble