Fig. 2
From: IL-33/ST2 axis of human amnion fibroblasts participates in inflammatory reactions at parturition
Distribution of IL-33 and ST2 in human amnion tissue. (A and B) Immunohistochemical staining of IL-33 (A) and ST2 (B) in human amnion showing the presence of IL-33 and ST2 in both epithelial cells and fibroblasts. (C and D) Comparison of IL33 and IL1RL1 mRNA abundance in human amnion fibroblasts and epithelial cells as determined with qRT-PCR. n = 3. (E and F) Comparison of IL-33 and ST2 protein abundance in human amnion fibroblasts and epithelial cells as determined with Western blotting. n = 3. The top panels of E and F are the representative immunoblots and the bottom panels of E and F are the average data. AE, amnion epithelial cells; AF, amnion fibroblasts. Data are mean ± SEM. Statistical analysis was performed with paired Student’s t-test. **p < 0.01, ***p < 0.001 vs. AE