Fig. 4

Effect of IL-33 on the expression of COX-2, IL-1β and IL-6 in human amnion fibroblasts. (A-C) Time-dependent (2, 4, 8, 12 and 24 h) induction of PTGS2, IL1B and IL6 mRNA expression by IL-33 (100 ng/mL). n = 3. (D-F) Concentration-dependent induction of PTGS2, IL1B and IL6 mRNA expression by IL-33 (50, 100 and 200 ng/mL; 8 h). n = 3. (G) Concentration-dependent induction of COX-2 protein by IL-33 (50, 100 and 200 ng/mL; 8 h). n = 4. The top panel is the representative immunoblots and the bottom panel is the average data. (H and I) Induction of PGE2 (H), IL-1β and IL-6 (I) secretion by IL-33 (100 ng/mL; 8 h). n = 7. Data are mean ± SEM. Statistical analysis was performed with one-way ANOVA test followed by the Newman-Keuls multiple comparison test (A-G) or paired Student’s t-test (H and I). *p < 0.05, **p < 0.01, ***p < 0.001 vs. 0 h or 0 ng/mL