Fig. 5

Phosphorylation of JNK, ERK1/2, p38 and NF-κB p65 by IL-33 in human amnion fibroblasts. (A–C) Time-dependent effects of IL-33 (100 ng/mL) on JNK (A), ERK1/2 (B), and p38 (C) phosphorylation. n = 4. (D–F) Effects of JNK inhibitor SP600125 (SP; 20 µM), ERK1/2 inhibitor PD98059 (PD; 20 µM) and p38 inhibitor SB203580 (SB; 10 µM) on IL-33 (100 ng/mL; 8 h)-induced NF-κB p65 phosphorylation. n = 3. Top panels are the representative immunoblots and bottom panels are the average data. Data are mean ± SEM. Statistical analysis was performed with one-way ANOVA test followed by the Newman-Keuls multiple comparison test. *p < 0.05, **p < 0.01 vs. 0 h or 0 ng/mL; #p < 0.05, ##p < 0.01, ###p < 0.001 vs. IL-33-treated group