Fig. 7

Role of ST2 in the induction of COX-2, IL-1β and IL-6 by IL-33 in human amnion fibroblasts. (A-H) Effects of siRNA-mediated knockdown of ST2 on the induction of COX-2, IL-1β and IL-6 by IL-33 (100 ng/mL; 8 h). A and E are the representative immunoblots. B and F are the knockdown efficiency of ST2 protein. n = 4–5. (I-L) Effects of siRNA-mediated knockdown of ST2 on the induction of the phosphorylation of JNK, ERK1/2, p38 and p65 by IL-33 (100 ng/mL; 1.5 h). n = 3–4. Top panels are the representative immunoblots and bottom panels are the average data. Data are mean ± SEM. Statistical analysis was performed with one-way ANOVA test followed by the Newman‐Keuls multiple comparison test. *p < 0.05, **p < 0.01, ***p < 0.001 vs. 0 ng/mL. #p < 0.05, ##p < 0.01, ###p < 0.001 vs. IL-33-treated groups