Fig. 2
CAMK4 overexpression increased viability, accelerated migration and invasion of HTR-8/SVneo cells under IR condition. A HTR-8/SVneo cells were pre-incubated with or without 10–6 mol/L insulin for 48 h, followed by stimulation with 10−7 mol/L insulin for 15 min. The protein levels of CAMK4 in HTR-8/SVneo cells were detected by western blot. B and C Insulin pre-incubation was performed 24 h after transfection of CAMK4 overexpression plasmid or empty vector. The protein and mRNA levels of CAMK4 in HTR-8/SVneo cells were detected by western blot (B) and real-time PCR (C), respectively. D The viability of HTR-8/SVneo cells was determined by CCK-8 assay. E and F The migratory capacity of HTR-8/SVneo cells was determined by wound healing assay. Scale bar = 200 μm (E). The wound width was measured at 0 h and 24 h post-scratching, and the migration rate was calculated (F). G and H The invasive capacity of HTR-8/SVneo cells was determined by matrigel-based transwell assay. Scale bar = 100 μm (H).The number of cells that invaded beyond the lower surface of the membrane was counted (G). Error bars depict thestandard deviation of the mean. IR, insulin resistance; −, no treatment; ns no significance, **p < 0.01, ***p < 0.001