Fig. 1
From: ERRα protects against sepsis-induced acute lung injury in rats
Overexpression of ERRα alleviates LPS-induced endothelial hyperpermeability in vitro. A. Representative immunofluorescence showing the expression of ERRα from LPS-treated PMVECs, observed by confocal microscopic images. Scale bar, 10 μm. B-C. RT-PCR (B), Western blot and quantitative analysis (C) of ERRα expression in Control and LPS groups. D-E. The levels of ERRα mRNA (D), ERRα protein and quantitative analysis (E) after transfected Ad-Vector and Ad-ERRα adenovirus. F. Comparison of endothelial permeability in three groups of cells after LPS treated 2, 6, 12 h. G. Representative confocal microscopic images showing the expression of Zo-1 in three groups. Scale bar, 10 μm. H. Western blot and quantitative analysis of ZO-1, Occludin, JAM-A, and VE-cadherin. Values are expressed as mean ± SD. ** P < 0.01, *P < 0.05 versus the Control or Vector group; #P < 0.05 versus the Vector + LPS group