Fig. 2
From: ERRα protects against sepsis-induced acute lung injury in rats
ERRα has a protective effect in LPS-induced apoptosis in PMVECs. A. The production of ROS in PMVECs using flow cytometry, and the shift to right of the peak represents an increase in ROS production. B. Representative blots and quantitative results of Sirt3 in three groups. C, G. TUNEL analysis of cells apoptosis in LPS-treated PMVECs transfected with Ad-ERRα adenovirus (C) or sh-ERRα lentivirus (G). the red nucleus is the positive. Scale bar, 50 μm. D, H. The apoptosis of cells stained with Annexin V and PI was determined by flow cytometry in LPS-treated PMVECs transfected with Ad-ERRα adenovirus (D) or sh-ERRα lentivirus (H). E-F, I-J. Representative blots and quantitative analysis showing the relative protein levels of apoptosis-associated proteins after transfected with Ad-ERRα adenovirus (E-F) or sh-ERRα lentivirus (I-J). *P < 0.05 versus the Control or Vector group; #P < 0.05 versus the Vector + LPS or LPS group