Fig. 3

ERRα is essential for autophagy activation in PRMVECs in response to LPS. A, D. Representative confocal microscopy images of LC3 in different groups (A, the overexpressed adenovirus transfection groups and D, the knockdown lentivirus transfection groups) of PMVECs infected with RFP-GFP-LC3 adenovirus for 24 h. The yellow puncta (solid arrow) represent autophagosomes and the red puncta (dashed arrow) represent autolysosomes. Scale bar, 10 μm. B, E. Representative immunofluorescence showing the expression of p62 after LPS-treated from different groups (B, the overexpressed adenovirus transfection groups and E, the knockdown lentivirus transfection groups). Scale bar, 10 μm. C, F. Representative blots and quantitative analysis showing the relative protein levels of autophagy-associated proteins in different groups (C, the overexpressed adenovirus transfection groups and F, the knockdown lentivirus transfection groups). *P < 0.05 versus the Control or Vector group; #P < 0.05 versus the Vector + LPS or LPS group