Fig. 3

Tan I suppressed ASC oligomerization. (A and B) BMDMs were cultured with LPS for 4 h, then incubated with DMSO or Tan I for 1 h, or BMDMs were first incubated with Tan I for 1 h, followed by LPS treatment for 4 h, protein level of NLRP3 and pro-IL-1β was determined by immunoblotting (A), ELISA was used to detect TNF-α secretion (B). (C-E) Immunoblotting of cross-linked pellets from LPS-primed BMDMs treated with Tan I before being stimulated by the indicated agonists. Data are shown as mean ± SEM from triplicates, *P < 0.05, **P < 0.01, NS: not significant (one-way ANOVA with Dunnett’s post hoc test)