Fig. 5

Tan I relieved LPS-induced septic shock and inhibited NLRP3 inflammasome activation in vivo. (A) Female mice (8 weeks) were intraperitoneally (i.p.) injected with vehicle, MCC950 (20 mg/kg), or Tan I (10, 20 mg/kg) respectively, 2 h later, administered with LPS (20 mg/kg, i.p.), then monitored for 72 h (n = 10). (B, C) Mice were injected intraperitoneally with 20 mg/kg Tan I, 20 mg/kg MCC950 for 2 h, followed by an injection of 20 mg/kg LPS for 4 h (n = 6). The levels of IL-1β in peritoneal lavage fluid (B) and serum (C) were analyzed by ELISA. Data are shown as mean ± SEM, ***P < 0.001 (log-rank test (A), one-way ANOVA with Dunnett’s post hoc test (B and C))