Fig. 6

Tan I ameliorated non-alcoholic steatohepatitis in mice. (A-D) Male C57BL/6 mice (8 weeks, 18-22 g) were fed MCD diet, while the control groups received MCS diet. The MCD and MCS fed mice were divided into groups (n = 6) that received vehicle, Tan I (20 mg/kg), or MCC950 (20 mg/kg) every day for 5 days, and subsequently 40 mg/kg via gavage every other day for six weeks. The liver morphology of mice in different treatment groups was shown, scale bar: 1 cm (A). Serum ALT and AST levels were determined by ELISA (B and C). Liver sections were stained with HE, Sirius Red, and Masson, scale bar: 200 μm (D). Data are shown as mean ± SEM, ***P < 0.001 (one-way ANOVA with Dunnett’s post hoc test)