Fig. 4

DEC2 regulates glycolysis in LN serum-treated MCs. A Diagram for the glycolytic function assay. B ECAR of the MCs was dynamically monitored along with the sequential addition of glucose, oligomycin and 2-DG. Multiple parameters including glycolysis, glycolytic capacity and glycolytic reserve can be calculated. C, D Both glycolysis level and glycolytic capacity significantly increased in the LN serum-treated MCs compared with those in controls, these increases in glycolysis level and glycolytic capacity can be partly reversed after knockdown of DEC2 in the MCs. E No statistical differences were found in the glycolytic reserve among groups. **P < 0.01 vs. control; ^##P < 0.01 vs. LN serum + Sh-NC