Fig. 3
From: NNAT is a novel mediator of oxidative stress that suppresses ER + breast cancer
NNAT regulates intracellular calcium in ER + breast cancer cells through EndoR calcium storage. A Confocal fluorescent imaging revealed NNAT (green) colocalization with EndoR and lysosome (red and yellow merged images). B Basal Ca2+ concentration in T47D and ZR75 CRISPR knockout of NNAT cell lines (NNAT CRISPR) (n = 30 per group, paired t-test, p < 0.001 vs. control). C EndoR Ca2+ release following knockout of NNAT (n ≥ 16 per group; paired t-test, **p < 0.01 vs. respective CRISPR control). D Basal Ca2+ concentration in T47D and ZR75 cell lines overexpressing NNAT (n = 30 per group, paired t-test, p < 0.001 vs. control). E Change in proliferative capacity in T47D and ZR75 breast cancer cell lines overexpressed NNAT deletion construct (wild-type, NNAT, and dER). (n = 9 per group; one-way ANOVA tests (Tukey post hoc test) *p < 0.05, ***p < 0.001 vs. GFP).