Fig. 4
From: NNAT is a novel mediator of oxidative stress that suppresses ER + breast cancer
ORAI but not TRPC3 inhibition reduces NNAT-mediated elevation in intracellular Ca2+concentration in breast cancer cells. The overexpression of NNAT promotes significantly higher Ca2+ release from EndoR of ZR75 breast cancer cells. (n = 30 per group; paired t-test, ***p < 0.001 vs. control GFP) (A). Intracellular Ca2+ levels in standard and overexpressing NNAT T47D and ZR75 cell lines in the presence or absence of ORAI (pyr6) (B) or TRPC3 (pyr3) (C) pyrazole compound inhibitors (n = 30 per group; Two-way ANOVA tests (factors: NNAT and inhibitor; Tukey post hoc test) ***p < 0.001)