Fig. 1

Sirt6 responds to changes in the HFD environment. A Confocal images of Iba1 (red) and Sirt6 (green) in the hypothalamus after 4 and 8 weeks of HFD. Arrowheads indicate representative cells showing Ibal-positive cells. Scale bars: 100 μm. BSirt6 mRNA expression level in microglia of male mice on a HFD for 24 weeks. Unpaired t-test. n = 6/group. C Sirt6 protein expression level in primary microglia of one-day-old neonatal mice after OA&PA treatment for 24 h. Unpaired t-test. n = 6/group. Data are presented as mean ± SEM, * p < 0.05, ** p < 0.01