Fig. 2

Microglia Sirt6 knockout mice are more prone to obesity after a 12-week HFD. A The growth curve of male mice on a HFD for 12 weeks. n = 7/group. B Liver-to-bodyweight ratio of male mice after a 12-week HFD. n = 7/group. C HE and oil red stain of liver cells of male mice after a 12-week HFD. Scale bars: 50 μm. D MicroCT image of iWAT and eWAT of male mice after a 12-week HFD. E Tissue weight of iWAT, eWAT, and BAT of male mice after a 12-week HFD. n = 7/group. F HE staining of iWAT, eWAT, and BAT of male mice after a 12-week HFD. Scale bars: 50 μm. G Serum insulin level in overnight fasted male mice after a 12-week HFD. n = 6–7/group. H Glucose tolerance test result of male mice after a 12-week HFD. n = 7/group. I Insulin tolerance test result of male mice after a 12-week HFD. n = 7/group. J Pyruvate tolerance test result of male mice after a 12-week HFD. n = 7/group. Data are presented as mean ± SEM, * p < 0.05, ** p < 0.01