Fig. 3

Regulation of food intake by microglia Sirt6 correlates with leptin function. A mRNA expression of leptin signalling in the hypothalamus of male mice under a HFD for 8 weeks. n = 8–9/group. B Fluorescence images of P-STAT3 (Ser727) in the hypothalamus of mice after injection of 1% BSA or leptin (1 µg/g) for 45 min. Scale bars: 100 μm. C Quantitative analysis of immunofluorescent intensity of P-STAT3 (Ser727) in the hypothalamus of mice after injection of 1% BSA or leptin (1 µg/g) for 45 min. n = 3–4/group. D Food intake change 1 h after leptin injection. n = 5–7/group. E Food intake change 2 days after leptin injection. n = 5/group. F Changes in body weight after leptin injection. n = 5/group. C, D Two-way ANOVA analysis followed by the Tukey post hoc test were performed. Data are presented as mean ± SEM, * p < 0.05, ** p < 0.01