Fig. 5

Sirt6 deficiency in microglia exacerbates high-fat diet-induced hypothalamic inflammation. A Immunofluorescent localization of Iba1 (red) and TNF-α (green) in hypothalamus. Arrowheads indicate representative cells showing co-localization. Scale bars: 100 μm. B TNF-α positive microglia counting from mice on a HFD for 12 weeks. n = 5–6/group. C mRNA expression of inflammatory factors in the hypothalamus of male mice on a HFD for 8 weeks. n = 8–12/group. Data are presented as mean ± SEM, * p < 0.05, ** p < 0.01