Fig. 6

Effect of Sirt6 on the inflammation of BV2 cells treated with OA&PA for 24 h. A Expression of M1-type microglia surface markers (Cd16, Cd86) and M2-type microglia surface markers (Ym1/2) in BV2 cells while Sirt6 was knocked down. B Expression of M1-type microglia surface markers (Cd16, Cd86) and M2-type microglia surface markers (Ym1/2) in BV2 cells while Sirt6 was over-expressed. C mRNA levels of Tnf-α, Il-6, and Il-1β in BV2 cells while Sirt6 was over-expressed. D Inflammatory cytokine (TNF-α, IL-6, and IL-1β) content in culture medium while Sirt6 was over-expressed. E, F Immunofluorescence images of CD68 and TNF-α while Sirt6 was over-expressed. Scale bars: 10 μm. G, H Quantitative analysis of immunofluorescent intensity of CD68 and TNF-α while Sirt6 was over-expressed. A-D, G-H Two-way ANOVA analysis was performed, followed by the Tukey post hoc test. n = 4–8/group. Data are presented as mean ± SEM, * p < 0.05, ** p < 0.01, *** p < 0.001