Fig. 7

Interaction of Sirt6 with NRF2. A Transcriptome screening for antioxidation. n = 2/group. B Overexpression of Sirt6 increases mRNA expression of antioxidant genes Nrf2, Ho-1, Gclc, and Gclm in BV2 Cells. n = 8–12/group. C Knockdown of Sirt6 decreases mRNA expression of antioxidant genes Nrf2, Ho-1, Gclc, and Gclm in BV2 Cells. n = 4/group. D Co-IP results verify that Sirt6 interacts with NRF2 in BV2 Cells. E Molecular docking of Sirt6 and NRF2 protein. F Effect of Sirt6 overexpression on NRF2 in BV2 Cells. G Immunofluorescence images of NRF2 while Sirt6 was over-expressed in BV2 Cells. Scale bars: 10 μm. H Quantitative analysis of immunofluorescent intensity of NRF2 while Sirt6 was over-expressed in BV2 Cells. n = 4/group. Data are presented as mean ± SEM, * p < 0.05, *** p < 0.001